UCSF

Purpose

Rapid chemical exchange can affect SNR and pH measurement accuracy for hyperpolarized pH imaging with [¹³ C]bicarbonate. The purpose of this work was to investigate chemical exchange effects on hyperpolarized imaging sequences to identify optimal sequence parameters for high SNR and pH accuracy.

Methods

Simulations were performed under varying rates of bicarbonate-CO₂ chemical exchange to analyze exchange effects on pH quantification accuracy and SNR under different sampling schemes. Four pulse sequences, including 1 new technique, a multiple-excitation 2D EPI (multi-EPI) sequence, were compared in phantoms using hyperpolarized [¹³ C]bicarbonate, varying parameters such as tip angles, repetition time, order of metabolite excitation, and refocusing pulse design. In vivo hyperpolarized bicarbonate-CO₂ exchange measurements were made in transgenic murine prostate tumors to select in vivo imaging parameters.

Results

Modeling of bicarbonate-CO₂ exchange identified a multiple-excitation scheme for increasing CO₂ SNR by up to a factor of 2.7. When implemented in phantom imaging experiments, these sampling schemes were confirmed to yield high pH accuracy and SNR gains. Based on measured bicarbonate-CO₂ exchange in vivo, a 47% CO₂ SNR gain is predicted.

Conclusion

The novel multi-EPI pulse sequence can boost CO₂ imaging signal in hyperpolarized ¹³ C bicarbonate imaging while introducing minimal pH bias, helping to surmount a major hurdle in hyperpolarized pH imaging.