Zhang, Jin; Li, Min; Zhang, Zhu; Zhu, Ronghui; Olcese, Riccardo; Stefani, Enrico; Toro, Ligia

doi:10.1016/j.mito.2016.08.017

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The mitochondrial BK_Ca channel cardiac interactome reveals BK_Ca association with the mitochondrial import receptor subunit Tom22, and the adenine nucleotide translocator.

2017

Published Web Location

https://doi.org/10.1016/j.mito.2016.08.017

Abstract

Mitochondrial BK_Ca channel, mitoBK_Ca, regulates mitochondria function in the heart but information on its protein partnerships in cardiac mitochondria is missing. A directed proteomic approach discovered the novel interaction of BK_Ca with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). The expressed protein partners co-immunoprecipitated and co-segregated into mitochondrial fractions in HEK293T cells. The BK_Ca 50 amino acid splice insert, DEC, facilitated BK_Ca interaction with ANT. Further, BK_Ca transmembrane domain was required for the association with both Tom22 and ANT. The results serve as a working framework to understand mitoBK_Ca import and functional relationships.

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The mitochondrial BKCa channel cardiac interactome reveals BKCa association with the mitochondrial import receptor subunit Tom22, and the adenine nucleotide translocator.

Published Web Location

The mitochondrial BK_Ca channel cardiac interactome reveals BK_Ca association with the mitochondrial import receptor subunit Tom22, and the adenine nucleotide translocator.