Guard cells are structures in the epidermis of plants that regulate gas exchange and water retention by controlling the opening and closing of the stomata. Guard cells can change configurations from a turgid state to a shrunken state and vice versa to control the aperture of stomatal pores. When plants detect the presence of abiotic stressors, such as high temperature, salinity, and carbon dioxide levels, the activation of different signal transduction cascades can cause changes in plant physiological responses and stomatal pore aperture. Abscisic Acid (ABA), a stress hormone in plants, is responsible for controlling gas exchange and water loss via regulating the opening and closing of the stomatal pore aperture. However, the gene expression and signaling cascade triggered by ABA that induces stomatal closure during stress responses have yet to be fully understood. Recent research has discovered that genes such as MAPKK Kinases (M3K), F-box, and Gibberellic- Acid Stimulated Arabidopsis (GASAs) play important roles in the ABA signal transduction pathway. Therefore, to figure out more information about the effect ABA has on the gene expression of regulatory components in guard cells, various data mining analyses were performed on these genes of interest. These analyses revealed that with the treatment of ABA, the gene expression of various Raf-like MAPKK Kinases (M3Ks) was significantly upregulated in guard cells. The increase of gene expression of these M3Ks could be linked to the stress- induced physiological responses in plants in the ABA signaling pathway. Phospho-proteomic analyses also identified a probable highly conserved phosphorylation site among F-box proteins and homologs.